Геном прокариот/Материалы:NC 005945

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   LOCUS       NC_005945            5228663 bp    DNA     circular BCT 03-APR-2006
   DEFINITION  Bacillus anthracis str. Sterne, complete genome.
   ACCESSION   NC_005945
   VERSION     NC_005945.1  GI:49183039
   KEYWORDS    .
   SOURCE      Bacillus anthracis str. Sterne
     ORGANISM  Bacillus anthracis str. Sterne
               Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus; Bacillus
               cereus group.
   REFERENCE   1  (bases 1 to 5228663)
     AUTHORS   Brettin,T.S., Bruce,D., Challacombe,J.F., Gilna,P., Han,C.,
               Hill,K., Hitchcock,P., Jackson,P., Keim,P., Longmire,J., Lucas,S.,
               Okinaka,R., Richardson,P., Rubin,E. and Tice,H.
     TITLE     Complete genome sequence of Bacillus anthracis Sterne
     JOURNAL   Unpublished
   REFERENCE   2  (bases 1 to 5228663)
     AUTHORS   .
     CONSRTM   NCBI Genome Project
     TITLE     Direct Submission
     JOURNAL   Submitted (11-SEP-2004) National Center for Biotechnology
               Information, NIH, Bethesda, MD 20894, USA
   REFERENCE   3  (bases 1 to 5228663)
     AUTHORS   Brettin,T.S., Bruce,D., Challacombe,J.F., Gilna,P., Han,C.,
               Hill,K., Hitchcock,P., Jackson,P., Keim,P., Longmire,J., Lucas,S.,
               Okinaka,R., Richardson,P., Rubin,E. and Tice,H.
     TITLE     Direct Submission
     JOURNAL   Submitted (12-JAN-2004) Joint Genome Institute, Department of
               Energy, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA
   COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
               NCBI review. The reference sequence was derived from AE017225.
               The Sterne strain of Bacillus anthracis is lacking the plasmid
               pXO2, resulting in an avirulent phenotype that maintains the main
               anthrax toxin. Prevention of anthrax in cattle can be accomplished
               by vaccination with living spores of the Sterne strain.  Most
               livestock vaccines in use throughout the world are derivatives of
               the live spore vaccine formulated by Sterne in 1937 and still use
               descendants of his strain 34F2 (Anthrax: the disease in relation to
               vaccines. Hambleton, P, Carnabm J, and Melling J. Vaccine 1984
               2:125-132 and Anthrax vaccines: past, present and future. Turnbull,
               PC. Vaccine 1991 9(8):533-539).   Plasmid and fosmid libraries were
               prepared at the Joint Genome Institute in Los Alamos (JGI-LANL),
               NM. Shotgun sequencing was performed at the JGI Production Genomics
               Facility (JGI-PGF) in Walnut Creek, CA. to a coverage of 16x.
               Finishing was performed at JGI-LANL starting with 39 contigs and 15
               scaffolds. Repetitive regions were identified, assembled and
               finished individually using a local software package called RepFin.
               Twenty four gaps were closed with primer walks and 15 by PCR. Gene
               predictions were performed using Glimmer and tRNAs were identified
               using tRNAScan-SE. Basic analysis of the gene predictions was
               performed by searching the coding sequence against the PFam,
               BLOCKS, and Prodom databases. For the most part, gene definitions
               were transferred directly from the TIGR Bacillus anthracis Ames
               GenBank records using a bi-directional best hit mapping between the
               two genomes. A total of 5415 features have been annotated on the
               sequence record.
               COMPLETENESS: full length.