Геном прокариот/Материалы:NC 008309

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   LOCUS       NC_008309            2007700 bp    DNA     circular BCT 16-AUG-2007
   DEFINITION  Haemophilus somnus 129PT, complete genome.
   ACCESSION   NC_008309
   VERSION     NC_008309.1  GI:113460149
   KEYWORDS    .
   SOURCE      Haemophilus somnus 129PT
     ORGANISM  Haemophilus somnus 129PT
               Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
               Pasteurellaceae; Histophilus.
   REFERENCE   1  (bases 1 to 2007700)
     AUTHORS   Challacombe,J.F., Duncan,A.J., Brettin,T.S., Bruce,D., Chertkov,O.,
               Detter,J.C., Han,C.S., Misra,M., Richardson,P., Tapia,R.,
               Thayer,N., Xie,G. and Inzana,T.J.
     TITLE     Complete genome sequence of Haemophilus somnus (Histophilus somni)
               strain 129Pt and comparison to Haemophilus ducreyi 35000HP and
               Haemophilus influenzae Rd
     JOURNAL   J. Bacteriol. 189 (5), 1890-1898 (2007)
      PUBMED   17172329
   REFERENCE   2  (bases 1 to 2007700)
     CONSRTM   NCBI Genome Project
     TITLE     Direct Submission
     JOURNAL   Submitted (30-AUG-2006) National Center for Biotechnology
               Information, NIH, Bethesda, MD 20894, USA
   REFERENCE   3  (bases 1 to 2007700)
     AUTHORS   Copeland,A., Lucas,S., Lapidus,A., Barry,K., Glavina del Rio,T.,
               Hammon,N., Dalin,E., Tice,H., Pitluck,S., Brettin,T.S., Bruce,D.,
               Challacombe,J.F., Chertkov,O., Detter,J.C., Gilna,P., Han,S.,
               Misra,M., Tapia,R., Thayer,N.N., Xie,G., Inzana,T.J., Duncan,A.J.,
               Siddaramppa,S. and Richardson,P.
     TITLE     Direct Submission
     JOURNAL   Submitted (07-AUG-2006) US DOE Joint Genome Institute, 2800
               Mitchell Drive B100, Walnut Creek, CA 94598-1698, USA
   COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
               NCBI review. The reference sequence was derived from CP000436.
               URL -- http://www.jgi.doe.gov
                      JGI Project ID: 2662200
                      Source DNA and bacteria available from Tom Inzana
                      Contacts: Tom Inzana (tinzana@vt.edu)
                                Paul Richardson (microbes@cuba.jgi-psf.org)
               Plasmid and fosmid libraries were prepared at the Joint Genome
               Institute (JGI) Production Genomics Facility (JGI-PGF) in Walnut
               Creek, CA. Shotgun sequencing was performed at the JGI-PGF. Large
               (40 kb), median (8 kb) and small (3 kb) insert random sequencing
               libraries were sequenced for this genome project with average
               success rate of 96% and average high-quality read lengths of 685
               nucleotides. After shotgun stage, reads were assembled with
               parallel phrap (High Performance Software, LLC). Possible
               mis-assemblies were corrected with Dupfinisher (unpublished, S.
               Han) or transposon bomb of bridging clones. Gaps between contigs
               were closed by editing, custom primer walks or PCR amplification.
               The completed genome sequence of H. somnus 129PT contains 59147
               reads, achieving an average of 18-fold sequence coverage per base
               with error rate less than 1 in 100,000. Gene predictions were
               obtained using Glimmer and tRNAs were identified using tRNAScan-SE.
               Basic analysis of the gene predictions was performed by comparing
               coding sequences against the PFam, BLOCKS, COGS and Prodom
               databases. Gene definitions and functional classes were added
               manually by a team of annotators at JGI-LANL, using BLAST results
               in addition to information from the basic analysis. A total of 1880
               features have been annotated on the sequence record.
                The JGI and collaborators endorse the principles for the
               distribution and use of large scale sequencing data adopted by the
               larger genome sequencing community and urge users of this data to
               follow them. It is our intention to publish the work of this
               project in a timely fashion and we welcome collaborative
               interaction on the project and analysis.
               (http://www.genome.gov/page.cfm?pageID=10506376).
               COMPLETENESS: full length.