Геном прокариот/Материалы:NC 009674

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   LOCUS       NC_009674            4087024 bp    DNA     circular BCT 25-JUL-2007
   DEFINITION  Bacillus cereus subsp. cytotoxis NVH 391-98, complete genome.
   ACCESSION   NC_009674
   VERSION     NC_009674.1  GI:152973854
   KEYWORDS    .
   SOURCE      Bacillus cereus subsp. cytotoxis NVH 391-98
     ORGANISM  Bacillus cereus subsp. cytotoxis NVH 391-98
               Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus; Bacillus
               cereus group.
   REFERENCE   1  (bases 1 to 4087024)
     AUTHORS   Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina
               del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S.,
               Foster,B., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N.,
               Kim,E., Richardson,P., Auger,S., Galleron,N., Sanchis,V., Gohar,M.,
               Broussole,V., Brillard,J., Guinebretiere,M.-H., Ehrlich,S.D.,
               Lereclus,D., Aymerich,S., Nguen-the,C. and Sorokin,A.
     CONSRTM   US DOE Joint Genome Institute
     TITLE     Complete sequence of chromosome of Bacillus cereus subsp. cytotoxis
               NVH 391-98
     JOURNAL   Unpublished
   REFERENCE   2  (bases 1 to 4087024)
     CONSRTM   NCBI Genome Project
     TITLE     Direct Submission
     JOURNAL   Submitted (24-JUL-2007) National Center for Biotechnology
               Information, NIH, Bethesda, MD 20894, USA
   REFERENCE   3  (bases 1 to 4087024)
     AUTHORS   Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina
               del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S.,
               Foster,B., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N.,
               Kim,E., Richardson,P., Auger,S., Galleron,N., Sanchis,V., Gohar,M.,
               Broussole,V., Brillard,J., Guinebretiere,M.-H., Ehrlich,S.D.,
               Lereclus,D., Aymerich,S., Nguen-the,C. and Sorokin,A.
     CONSRTM   US DOE Joint Genome Institute
     TITLE     Direct Submission
     JOURNAL   Submitted (10-JUL-2007) US DOE Joint Genome Institute, 2800
               Mitchell Drive B100, Walnut Creek, CA 94598-1698, USA
   COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
               NCBI review. The reference sequence was derived from CP000764.
               URL -- http://www.jgi.doe.gov
               JGI Project ID: 4000557
               Source DNA and bacteria available from Alexei Sorokin
               (alexei.sorokine@jouy.inra.fr)
               Contacts: Alexei Sorokin (alexei.sorokine@jouy.inra.fr)
                         Paul Richardson (microbes@cuba.jgi-psf.org)
               Quality assurance done by JGI-Stanford
               Finishing done by JGI-PGF
               Finished microbial genomes have been curated to close all gaps with
               greater than 98% coverage of a least two independent clones. Each
               base pair has a minumum q (quality) value of 30 and the total error
               rate is less than one per 50000.
               The JGI and collaborators endorse the principles for the
               distribution and use of large scale sequencing data adopted by the
               larger genome sequencing community and urge users of this data to
               follow them. It is our intention to publish the work of this
               project in a timely fashion and we welcome collaborative
               interaction on the project and analysis.
               (http://www.genome.gov/page.cfm?pageID=10506376)
               Additional notes:
               Bacillus cereus NVH 398-91 was originally isolated as a contaminant
               of vegetable puree in a retirement house in Essone (France) by
               Marie-Laure De Buyser (AFSSA, France). The strain was identified as
               a cause of a severe food poisoning case. It was further
               characterized biochemically and a novel cytotoxin K was identified
               as a major cause of epithelial cell lysis leading to necrotic
               enteritis (Lund et al, Mol Microbiol, 2000, 38, 254). Although the
               strain was identified as Bacillus cereus, it appeared to be
               different from all known strains. Detailed phylogenetic studies
               using MLST approach placed the strain rather far from main strain
               clusters, but confirmed that the strain belongs to the B. cereus
               group (Sorokin et al, Appl Env Microbiol, 2006, 72, 1569). Genomic
               sequence clearly indicated that the strain is very different from
               other representatives of the group and should be assigned a new
               species status, for which we propose a name Bacillus cytotoxis.
               However, assigning of the novel species status is hampered by the
               fact that only one such strain is described at the moment of
               genomic sequencing being done (2005).
               COMPLETENESS: full length.